Transfection is a powerful analytical tool enabling study of the function of genes and gene
products in cells. The transfection methods are broadly classified into three groups; …

There has been significant interest in developing cell membrane-coated nanoparticles due
to their unique abilities of biomimicry and biointerfacing. As the technology progresses, it …

We provide protocols for preparing low-density dissociated-cell cultures of hippocampal
neurons from embryonic rats or mice. The neurons are cultured on polylysine-treated …

Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …

This protocol describes a high-efficiency Ca 2+-phosphate transfection method with low cell
toxicity. The Ca 2+-phosphate transfection method is widely used in transfecting neurons …

The establishment of a polarized morphology is an essential step in the differentiation of
neurons with a single axon and multiple dendrites. In cultured rat hippocampal neurons, one …

The transfection of nucleic acids into cells is crucial for the study of many aspects of
neuronal cell biology. These include investigating gene and protein function by knocking …

Gene delivery as a promising and valid tool has been used for treating many serious
diseases that conventional drug therapies cannot cure. Due to the advancement of physical …

Glass micropipettes, atomic force microscope tips and nanoneedles can be used to
interrogate cells, but these devices either have conical geometries that can damage cells …

Coated calcium phosphate nanoparticles were prepared for cell transfection. A calcium
phosphate nanoparticle served as core which was then coated with DNA for colloidal …