Esterification is one of the most important reactions in organic synthesis and industrial
applications. The applications of lipases in esterification are increasingly widespread and …

Fructosyltransferase is a key enzyme in fructo-oligosaccharide production, while the highly
demanding conditions of industrial processes may reduce its stability and activity. This study …

The narrow substrate scope limits the wide industrial application of enzymes. Here, we
successfully broadened the substrate scope of a nitrile hydratase (NHase) through mutation …

Abstract Nitrile hydratase (NHase; EC 4.2. 1.84) is widely used to synthesize the
corresponding amides from nitriles, which is the most successful green biocatalyst …

Pullulanases are important starch-debranching enzymes that mainly hydrolyze the α-1, 6-
glycosidic linkages in pullulan, starch, and oligosaccharides. Nevertheless, their practical …

Owing to zero-calorie, high-intensity sweetness and good taste profile, the plant-derived
sweetener rebaudioside D (Reb D) has attracted great interest to replace sugars. However …

Nitrile hydratase (NHase), an excellent biocatalyst, has been widely used for the production
of amides, but the exothermic hydration reaction leads to its rapid inactivation, hindering its …

Amides are an important type of synthetic intermediate used in the chemical, agrochemical,
pharmaceutical, and nutraceutical industries. The traditional chemical process of converting …

Ethyl carbamate (EC) is classified as a Class 2A carcinogen, and is present in various
fermented foods, posing a threat to human health. Urethanase (EC 3.5. 1.75) can catalyze …

Transcription factor (TF)-based biosensors are expected to serve as powerful tools for the
high-throughput screening of biocatalytic systems; however, most of them respond to ligands …