Progress in structural biology research has led to a high demand for powerful and yet
complementary analytical tools for structural characterization of proteins and protein …

Proteomics is the large scale study of protein structure and function from biological systems
through protein identification and quantification.“Shotgun proteomics” or “bottom-up …

The growing interest in advancing tandem mass spectrometry strategies for top-down
proteomics has motivated efforts to optimize ion activation strategies for intact proteins …

Fragmentation trends of large peptides were characterized by five activation methods,
including HCD, ETD, EThcD, 213 nm UVPD, and 193 nm UVPD. Sequence coverages and …

Ultraviolet photodissociation (UVPD) is a powerful and rapidly developing method in top-
down proteomics. Sequence coverages can exceed those obtained with collision-and …

Analysis of native-like protein structures in the gas phase via native mass spectrometry and
auxiliary techniques has become a powerful tool for structural biology applications. In …

Crosslinking mass spectrometry provides pivotal information on the structure and interaction
of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of …

Ultraviolet photodissociation (UVPD) has recently been introduced as an ion activation
method for the determination of single-residue deuterium levels in H/D exchange tandem …

The analysis of dissociation products of disulfide bonds in peptide sequences has been
increasingly applied in various fields. Ion dissociation is typically performed by tandem mass …

This dissertation is focused on the development of top-down mass spectrometry methods for
characterization of proteins and nucleic acids, including investigations into tandem mass …