Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martínez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

A Currin, N Swainston, PJ Day, DB Kell - Chemical Society Reviews, 2015 - pubs.rsc.org
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Enzyme engineering and its industrial applications

I Victorino da Silva Amatto… - Biotechnology and …, 2022 - Wiley Online Library
Recently, there has been an increase in the demand for enzymes with modified activity,
specificity, and stability. Enzyme engineering is an important tool to meet the demand for …

Directed evolution 2.0: improving and deciphering enzyme properties

F Cheng, L Zhu, U Schwaneberg - Chemical Communications, 2015 - pubs.rsc.org
Directed evolution has matured to a routinely applied algorithm to tailor enzyme properties
to meet the demands in various applications. In order to free directed enzyme evolution from …

Thermostable phytase in feed and fuel industries

UM Vasudevan, A Jaiswal, S Krishna, A Pandey - 2019 - arrow.tudublin.ie
Phytase with wide ranging biochemical properties has long been utilized in a multitude of
industries, even so, thermostability plays a crucial factor in choosing the right phytase in a …

Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents

X Wang, Y Sheng, H Cui, J Qiao, Y Song… - Angewandte …, 2024 - Wiley Online Library
Deep eutectic solvents (DESs), heralded for their synthesis simplicity, economic viability,
and reduced volatility and flammability, have found increasing application in biocatalysis …

Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

L Navone, T Vogl, P Luangthongkam, JA Blinco… - Biotechnology for …, 2021 - Springer
Background Phytases are widely used commercially as dietary supplements for swine and
poultry to increase the digestibility of phytic acid. Enzyme development has focused on …

Microbial phytase: impact of advances in genetic engineering in revolutionizing its properties and applications

MV Ushasree, K Shyam, J Vidya, A Pandey - Bioresource technology, 2017 - Elsevier
Phytases are enzymes that increase the availability of phosphorous in monogastric diet and
reduces the anti-nutrition effect of phytate. This review highlights contributions of …

Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries

J Yang, AJ Ruff, M Arlt… - Biotechnology and …, 2017 - Wiley Online Library
During the last decade, directed evolution has become a standard protein engineering
strategy to reengineer proteins for industrial applications under high stress conditions (eg …

[HTML][HTML] Biotechnological production of food-grade polyphosphate from deoiled seeds and bran

KR Herrmann, J Fees, JJ Christ, I Hofmann… - EFB Bioeconomy …, 2023 - Elsevier
Agricultural products, have a high phytate content that has a hidden potential as a
renewable source of phosphate. We present the first biotechnological route for the …