Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment
that mediates the transport of proteins from the cytoplasm into the nucleus. This NLS …

Nuclear pore complexes (NPCs) perforate the nuclear envelope and serve as the primary
transport gates for molecular exchange between nucleus and cytoplasm. Stripping the …

Methods for the targeted disruption of protein function have revolutionized science and
greatly expedited the systematic characterization of genes. Two main approaches are …

Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …

Nanobodies are single-domain antibodies derived from the variable regions of Camelidae
atypical immunoglobulins. They show promise as high-affinity reagents for research …

The WD40 domain exhibits a β-propeller architecture, often comprising seven blades. The
WD40 domain is one of the most abundant domains and also among the top interacting …

Internal membrane bound structures sequester all genetic material in eukaryotic cells. The
most prominent of these structures is the nucleus, which is bounded by a double membrane …

Much of life's essential molecular machinery consists of large protein assemblies that
currently pose challenges for structure determination. A prominent example is the nuclear …

Although the nuclear pore complex (NPC) is best known for its primary function as the key
regulator of molecular traffic between the cytoplasm and the nucleus, a growing body of …

To understand the structure and function of large molecular machines, accurate knowledge
of their stoichiometry is essential. In this study, we developed an integrated targeted …