Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

Nature uses a limited, conservative set of amino acids to synthesize proteins. The ability to
genetically encode an expanded set of building blocks with new chemical and physical …

Polyploidy is a key mechanism of genome evolution and speciation, particularly in plants.
Many aspects of polyploidy have been elucidated with the tools that have become available …

Our understanding of the complex molecular processes of living organisms at the molecular
level is growing exponentially. This knowledge, together with a powerful arsenal of tools for …

Conspectus Chemical tools are transforming our understanding of biomolecules and living
systems. Included in this group are bioorthogonal reagents–functional groups that are inert …

Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing
the subtle behavior of the protein and assessing its cellular function. Over the last decade …

The vasculature is a key component of the tissue microenvironment. Traditionally known for
its role in providing nutrients and oxygen to surrounding cells, the vasculature is now also …

Fibrillar proteins are principal components of extracellular matrix (ECM) that confer
mechanical properties to tissues. Fibrosis can result from wound repair in nearly every tissue …

The ability to assign cellular origin to low-abundance secreted factors in extracellular
vesicles (EVs) would greatly facilitate the analysis of paracrine-mediated signaling. Here, we …

Cellular proteins are dynamically regulated in response to environmental stimuli.
Conventional proteomics compares the entire proteome in different cellular states to identify …