The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

The identification and characterization of protein interactions is a key topic in current life
science research; a huge variety of methodologies have been established in recent years to …

Existing protein tagging and detection methods are powerful but have drawbacks. Split
protein tags can perturb protein solubility,,, or may not work in living cells,,. Green …

We have studied the naturally split α subunit of the DNA polymerase III (DnaE) intein from
Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent …

In this study we developed an inducible synthetic renilla luciferase protein-fragment-assisted
complementation-based bioluminescence assay to quantitatively measure real time protein …

In this study we have developed bioluminescence-imaging strategies to noninvasively and
quantitatively image protein—protein interactions in living mice by using a cooled charge …

Abstract MicroRNAs (miRNAs) are short,~ 22 nucleotide length RNAs that perform gene
regulation. Recently, miRNA has been shown to be linked with the onset of cancer and other …

The use of small molecules that turn specific proteins on or off provides a level of temporal
control that is difficult to achieve using standard genetic approaches. Consequently, the …

Protein splicing is a naturally occurring process in which an intervening intein domain
excises itself out of a precursor polypeptide in an autocatalytic fashion with concomitant …

Artificial molecular switches that modulate protein activities in response to synthetic small
molecules would serve as tools for exerting temporal and dose-dependent control over …