Omics-based technologies have revolutionized our understanding of the coding potential of
the genome. In particular, these studies revealed widespread unannotated open reading …

Modern genome-scale methods that identify new genes, such as proteogenomics and
ribosome profiling, have revealed, to the surprise of many, that overlap in genes, open …

Abstract Ribosome profiling (Ribo-Seq) has proven transformative for our understanding of
the human genome and proteome by illuminating thousands of noncanonical sites of …

Messenger RNA (mRNA) stability and translational efficiency are two crucial aspects of the
post-transcriptional process that profoundly impact protein production in a cell. While it is …

Translation is pervasive outside of canonical coding regions, occurring in long noncoding
RNAs, canonical untranslated regions and introns,,–, especially in ageing …

Recent proteogenomic studies revealed extensive translation outside of annotated protein
coding regions, such as non-coding RNAs and untranslated regions of mRNAs. This non …

Short ORFs (sORFs), that is, occurrences of a start and stop codon within 100 codons or
less, can be found in organisms of all domains of life, outnumbering annotated protein …

Cellular senescence constitutes a generally irreversible proliferation barrier, accompanied
by macromolecular damage and metabolic rewiring. Several senescence types have been …

Microproteins (MPs) are a potentially rich source of uncharacterized metabolic regulators.
Here, we use ribosome profiling (Ribo-seq) to curate 3,877 unannotated MP-encoding small …

A crucial step in functional genomics is identifying actively translated ORFs and linking them
to biological functions. The challenge lies in identifying short ORFs, as their identification is …