Contents Flow cytometry has become an important technique in sperm evaluation and is
increasingly used both for routine assessment and for research in veterinary science. We …

Oxidative stress generates a large amount of reactive oxygen species (ROS) and affects
sperm quality via damaging sperm DNA and compromising the intracellular homeostasis in …

Apoptosis is a complex and finely controlled cell death process of great relevance in tissue
homeostasis and pathogenesis. The majority of classical apoptotic features can be …

OBJECTIVE: To compare the effects of density-gradient centrifugation and swim-up on
sperm apoptosis by using a multiparameter flow cytometric method. DESIGN: Autocontrolled …

OBJECTIVE: To examine simultaneously the levels of hydrogen peroxide (H2O2) and
superoxide (O2−•) using chemiluminescence and flow cytometry. DESIGN: Prospective …

Flow cytometry becomes a common method for analysis of spermatozoa quality. Standard
sperm characteristics such as viability, acrosome and chromatin integrity, oxidative damage …

In this article we describe the development of a microfluidic chip to determine the
concentration of spermatozoa in semen, which is a main quality parameter for the fertility of a …

Caspases are central components in the apoptosis signaling cascade. The family of cysteine
proteases transduces and enhances the apoptosis signal, and activation of effector …

There is a current need for new biomarkers of spermatozoa quality, that consistently and
correctly identify spermatozoa that will successfully contribute to subsequent embryo …

Nitric oxide (NO) is a highly reactive free radical that plays a key role in intra-and
intercellular signaling. Production of radical oxygen species and an apoptotic-like …