Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks

R Nyaruaba, C Mwaliko, D Dobnik… - Clinical Microbiology …, 2022 - Am Soc Microbiol
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health …

[HTML][HTML] Medical diagnostic value of digital PCR (dPCR): A systematic review

S Nazir - Biomedical Engineering Advances, 2023 - Elsevier
Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute
quantification of target nucleic acids. dPCR got attention as a precise quantification tool in …

SARS‐CoV‐2 in digital era: Diagnostic techniques and importance of nucleic acid quantification with digital PCRs

N Aravind Kumar, S Aradhana, Harleen… - Reviews in Medical …, 2023 - Wiley Online Library
Studies related to clinical diagnosis and research of SARS‐CoV‐2 are important in the
current pandemic era. Although molecular biology has emphasised the importance of …

Integration of RT-LAMP and microfluidic technology for detection of SARS-CoV-2 in wastewater as an advanced point-of-care platform

A Donia, M Furqan Shahid, S Hassan, R Shahid… - Food and Environmental …, 2022 - Springer
Abstract Development of lab-on-a-chip (LOC) system based on integration of reverse
transcription loop-mediated isothermal amplification (RT-LAMP) and microfluidic technology …

Evolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis of Coronavirus Disease

SF Khan, P Rathod, VK Gupta, PB Khedekar… - Analytical …, 2024 - ACS Publications
Severe acute respiratory syndrome Coronavirus 2, or SARS-CoV-2, previously known as
2019-CoV, is an encapsulated, positive-sense, single-stranded genomic RNA virus (+ …

Strategy to develop and evaluate a multiplex RT-ddPCR in response to SARS-CoV-2 genomic evolution

LAE Van Poelvoorde, M Gand, MA Fraiture… - Current Issues in …, 2021 - mdpi.com
The worldwide emergence and spread of severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) since 2019 has highlighted the importance of rapid and reliable diagnostic …

Omicron (B. 1.1. 529) SARS-CoV-2 viral load among nasopharyngeal and oral samples compared to other variants of concern and impact on diagnostic testing …

N Matic, CF Lowe, G Ritchie… - Clinical …, 2022 - clinicalmicrobiologyandinfection …
With the rapid wave of Omicron (B. 1.1. 529) SARS-CoV-2 cases detected worldwide and
the significant mutation profile of this variant of concern (VOC), questions have been raised …

A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

K Wagner, P Fox, E Gordon, W Hahn, K Olsen… - Scientific Reports, 2023 - nature.com
In response to the SARS-CoV-2 pandemic, we developed am ulti p lexed, p aired-p ool
droplet digital P CR (MP4) screening assay. Key features of our assay are the use of …

Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

DW Weisgerber, K Langer, V Servellita, P Xu, CY Chiu… - Lab on a Chip, 2023 - pubs.rsc.org
Viral load quantitation is useful in clinical point-of-care settings to assess the status of
patients with infectious disease, track response to treatment, and estimate infectiousness …

Development of a reverse-transcription droplet digital PCR method for quantitative detection of Cucumber green mottle mosaic virus

Y Tian, J Fei, J Luo, L Chen, J Ye, W Du, C Yu - Heliyon, 2023 - cell.com
Cucumber green mottle mosaic virus (CGMMV) is a re-emerging threat to the production of
greenhouse cucumber and other Cucurbitaceae crops worldwide. This seed-borne virus can …