Highly sensitive SERS quantification of the oncogenic protein c-Jun in cellular extracts

L Guerrini, E Pazos, C Penas… - Journal of the …, 2013 - ACS Publications
A surface-enhanced Raman scattering (SERS)-based sensor was developed for the
detection of the oncoprotein c-Jun at nanomolar levels. c-Jun is a member of the bZIP (basic …

Advances in lanthanide‐based luminescent peptide probes for monitoring the activity of kinase and phosphatase

E Pazos, ME Vázquez - Biotechnology journal, 2014 - Wiley Online Library
Signaling pathways based on protein phosphorylation and dephosphorylation play critical
roles in the orchestration of complex biochemical events and form the core of most signaling …

Luminescent lanthanide metallopeptides for biomolecule sensing and cellular imaging

R Sánchez-Fernández, I Obregon-Gomez… - Chemical …, 2024 - pubs.rsc.org
Lanthanide ions display unique luminescent properties that make them particularly attractive
for the development of bioprobes, including long-lived excited states that allow the …

Luminescent peptide/lanthanide (III) complex conjugates with push–pull antennas: application to one-and two-photon microscopy imaging

JH Choi, G Fremy, T Charnay, N Fayad… - Inorganic …, 2022 - ACS Publications
Lanthanide (III)(Ln3+) complexes feature desirable luminescence properties for cell
microscopy imaging, but cytosolic delivery of Ln3+ complexes and their use for 2P imaging …

Strand displacement in coiled‐coil structures: Controlled induction and reversal of proximity

K Gröger, G Gavins, O Seitz - Angewandte Chemie, 2017 - Wiley Online Library
Coiled‐coil peptides are frequently used to create new function upon the self‐assembly of
supramolecular complexes. A multitude of coil peptide sequences provides control over the …

Luminescent Ln(III)-Metallopeptide Sensors for Monitoring Pseudomonas aeruginosa Elastase B Activity in Complex Biological Media

R Sánchez-Fernández, M Sandá-Ares, N Lamas… - ACS …, 2024 - ACS Publications
The detection and monitoring of Pseudomonas aeruginosa and its virulence factors, such as
the LasB protease, are crucial for managing bacterial infections. Traditional fluorescent …

Straightforward access to bisbenzamidine DNA binders and their use as versatile adaptors for DNA-promoted processes

MI Sánchez, O Vázquez, J Martínez-Costas… - Chemical …, 2012 - pubs.rsc.org
Bisbenzamidines are an important family of minor groove DNA-binding agents. We present
a one-step synthesis of aromatic aza-bisbenzamidines that allows straightforward and …

Equipment-free and visualized biosensor for transcription factor rapid assay based on dopamine-functionalized cellulose paper

H Lin, X Wang, J Wu, H Li, F Li - Journal of Materials Chemistry B, 2019 - pubs.rsc.org
A cellulose paper-based biosensor was developed for the equipment-free and naked-eye
detection of a transcription factor (TF) using dopamine as the signal-responsive material …

Detection of phosphorylation states by intermolecular sensitization of lanthanide–peptide conjugates

E Pazos, M Goličnik, JL Mascareñas… - Chemical …, 2012 - pubs.rsc.org
The luminescence of a designed peptide equipped with a coordinatively-unsaturated
lanthanide complex is modulated by the phosphorylation state of a serine residue in the …

A folding-based approach for the luminescent detection of a short RNA hairpin

C Penas, E Pazos, JL Mascarenas… - Journal of the American …, 2013 - ACS Publications
We report the rational design of a 20-mer basic peptide, derived from the transcriptional
antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA [Tb3+] …