Abstract Nuclear DNA topoisomerase I (TOP1) is an essential human enzyme. It is the only
known target of the alkaloid camptothecin, from which the potent anticancer agents …

DNA topoisomerases constitute a large family of enzymes that are essential for all domains
of life. Although they share general reaction chemistry and the capacity to govern DNA …

R-loops comprise an RNA/DNA hybrid and displaced single-stranded DNA. They play
important biological roles and are implicated in pathology. Even so, proteins recognizing …

We report a mechanism through which the transcription machinery directly controls
topoisomerase 1 (TOP1) activity to adjust DNA topology throughout the transcription cycle …

Transcription and splicing must proceed over genomic distances of hundreds of kilobases in
many human genes. However, the rates and mechanisms of these processes are poorly …

Background Co-transcriptional R-loops are abundant non-B DNA structures in mammalian
genomes. DNA Topoisomerase I (Top1) is often thought to regulate R-loop formation owing …

Neutrophils are short-lived innate immune cells. These cells respond quickly to stimuli, and
die within minutes to hours; the relevance of DNA transcription in dying neutrophils remains …

Transcription has the capacity to mechanically modify DNA topology, DNA structure and
nucleosome arrangement. Resulting from ongoing transcription, these modifications in turn …

Coupling between transcription and RNA processing is a key gene regulatory mechanism.
Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation …

Topoisomerase I (TOP1) inhibitors applied in cancer therapy such as topotecan and
irinotecan are derivatives of the natural alkaloid camptothecin (CPT). The mechanism of …