Liquid biopsies come of age: towards implementation of circulating tumour DNA

JCM Wan, C Massie, J Garcia-Corbacho… - Nature Reviews …, 2017 - nature.com
Improvements in genomic and molecular methods are expanding the range of potential
applications for circulating tumour DNA (ctDNA), both in a research setting and as a'liquid …

dPCR: a technology review

PL Quan, M Sauzade, E Brouzes - Sensors, 2018 - mdpi.com
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

[HTML][HTML] Noninvasive prenatal screening for fetal aneuploidy, 2016 update: a position statement of the American College of Medical Genetics and Genomics

AR Gregg, BG Skotko, JL Benkendorf, KG Monaghan… - Genetics in …, 2016 - Elsevier
Disclaimer: This statement is designed primarily as an educational resource for clinicians to
help them provide quality medical services. Adherence to this statement is completely …

Analysis of circulating tumor DNA to monitor metastatic breast cancer

SJ Dawson, DWY Tsui, M Murtaza… - … England Journal of …, 2013 - Mass Medical Soc
Background The management of metastatic breast cancer requires monitoring of the tumor
burden to determine the response to treatment, and improved biomarkers are needed …

Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA

T Forshew, M Murtaza, C Parkinson, D Gale… - Science translational …, 2012 - science.org
Plasma of cancer patients contains cell-free tumor DNA that carries information on tumor
mutations and tumor burden. Individual mutations have been probed using allele-specific …

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

JF Huggett, CA Foy, V Benes, K Emslie… - Clinical …, 2013 - academic.oup.com
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …

Application of digital PCR for public health-related water quality monitoring

A Tiwari, W Ahmed, S Oikarinen, SP Sherchan… - Science of the Total …, 2022 - Elsevier
Digital polymerase chain reaction (dPCR) is emerging as a reliable platform for quantifying
microorganisms in the field of water microbiology. This paper reviews the fundamental …

Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification

LB Pinheiro, VA Coleman, CM Hindson… - Analytical …, 2012 - ACS Publications
Droplet digital polymerase chain reaction (ddPCR) is a new technology that was recently
commercialized to enable the precise quantification of target nucleic acids in a sample …

Considerations for digital PCR as an accurate molecular diagnostic tool

JF Huggett, S Cowen, CA Foy - Clinical chemistry, 2015 - academic.oup.com
Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …

Maternal plasma DNA sequencing reveals the genome-wide genetic and mutational profile of the fetus

YMD Lo, KCA Chan, H Sun, EZ Chen, P Jiang… - Science translational …, 2010 - science.org
Cell-free fetal DNA is present in the plasma of pregnant women. It consists of short DNA
fragments among primarily maternally derived DNA fragments. We sequenced a maternal …