Sequencing technologies have placed a wide range of genomic analyses within the
capabilities of many laboratories. However, sequencing costs often set limits to the amount …

A comprehensive understanding of host–pathogen interactions requires a knowledge of the
associated gene expression changes in both the pathogen and the host. Traditional, probe …

De novo assembly of RNA-seq data enables researchers to study transcriptomes without the
need for a genome sequence; this approach can be usefully applied, for instance, in …

Bacteria respond to changes in their environment with specific transcriptional programmes,
but even within genetically identical populations these programmes are not homogenously …

Bacterial transcriptional networks consist of hundreds of transcription factors and thousands
of promoters. However, the true complexity of transcription in a bacterial pathogen and the …

The transcriptome is a powerful proxy for the physiological state of a cell, healthy or
diseased. As a result, transcriptome analysis has become a key tool in understanding the …

While the model organism Escherichia coli has been the subject of intense study for
decades, the full complement of its RNAs is only now being examined. Here we describe a …

The human body is constantly exposed to microorganisms, which entails manifold
interactions between human cells and diverse commensal or pathogenic bacteria. The …

Although RNA-seq is a powerful tool, the considerable time and cost associated with library
construction has limited its utilization for various applications. RNAtag-Seq, an approach to …

Salmonella enterica serovar Typhimurium is arguably the world's best-understood bacterial
pathogen. However, crucial details about the genetic programs used by the bacterium to …