Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and
organismal scales, is encoded through a combination of genetic and epigenetic factors in …

CRISPR technologies have revolutionized research areas ranging from fundamental
science to translational medicine. CRISPR-based genetic screens offer a powerful platform …

Combinatorial CRISPR-Cas screens have advanced the mapping of genetic interactions,
but their experimental scale limits the number of targetable gene combinations. Here, we …

Simple Summary High-throughput CRISPR screening provides an unbiased way for
functional genomic studies in hematological malignancies. This approach has been used to …

Genome editing, or genetic modification, is a form of genetic engineering that replicates,
extracts, modifies, or replaces DNA in living cells. Once investigators can sequence and …

Single-cell CRISPR screens are the most promising biotechnology for mapping regulatory
elements to their target genes at genome-wide scale. However, the analysis of these …

The CRISPR/Cas9 genome editing system has been one of the greatest scientific
discoveries in the last decade. The highly efficient and precise editing ability of this …

Results Analysis challenges. The original analysis by Gasperini et al 11 was carried out
using Monocle2 30, whose differential expression analysis is motivated by DESeq2 28. The …

In this work, we explore the intersection of in situ sequencing, neural recording, and CRISPR
screens. An intracellular technology is outlined for encoding neural activity in the form of …