Synthesis of anisotropic hydrogels and their applications

K Sano, Y Ishida, T Aida - Angewandte Chemie International …, 2018 - Wiley Online Library
Owing to their water‐rich structures, which are similar to those of biological tissues,
hydrogels have long been regarded as promising scaffolds for artificial tissues and organs …

Exploring free-energy landscapes of intrinsically disordered proteins at atomic resolution using NMR spectroscopy

MR Jensen, M Zweckstetter, J Huang… - Chemical …, 2014 - ACS Publications
The last 15 years have seen a paradigm shift in our understanding of protein biochemistry,
with the realization that an unexpectedly high fraction of the human genome codes for …

Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate

EB Bertelsen, L Chang, JE Gestwicki… - Proceedings of the …, 2009 - National Acad Sciences
DnaK is the canonical Hsp70 molecular chaperone protein from Escherichia coli. Like other
Hsp70s, DnaK comprises two main domains: a 44-kDa N-terminal nucleotide-binding …

Unfolded proteins and protein folding studied by NMR

HJ Dyson, PE Wright - Chemical reviews, 2004 - ACS Publications
Preparation of biological macromolecules in the pure state requires that cells be disrupted,
releasing and mixing the contents. Only the most stable and highly structured molecules can …

Magnetic susceptibility in paramagnetic NMR

I Bertini, C Luchinat, G Parigi - Progress in Nuclear Magnetic Resonance …, 2002 - Elsevier
1. Introduction.................................................................. 250 2. Magnetic moments,
magnetization, magnetic susceptibility................................ 250 2.1. The Curie …

DNA-nanotube-induced alignment of membrane proteins for NMR structure determination

SM Douglas, JJ Chou, WM Shih - Proceedings of the …, 2007 - National Acad Sciences
Membrane proteins are encoded by 20–35% of genes but represent< 1% of known protein
structures to date. Thus, improved methods for membrane-protein structure determination …

Unusual architecture of the p7 channel from hepatitis C virus

B OuYang, S Xie, MJ Berardi, X Zhao, J Dev, W Yu… - Nature, 2013 - nature.com
The hepatitis C virus (HCV) has developed a small membrane protein, p7, which remarkably
can self-assemble into a large channel complex that selectively conducts cations,,,. We …

Protein dynamics and function from solution state NMR spectroscopy

M Kovermann, P Rogne, M Wolf-Watz - Quarterly reviews of …, 2016 - cambridge.org
It is well-established that dynamics are central to protein function; their importance is
implicitly acknowledged in the principles of the Monod, Wyman and Changeux model of …

Residual dipolar couplings in structure determination of biomolecules

JH Prestegard, CM Bougault, AI Kishore - Chemical reviews, 2004 - ACS Publications
The use of residual dipolar couplings (RDCs) in the analysis of biomolecular structure and
dynamics has expanded rapidly since its potential as a source of structural information on …

Application of anisotropic NMR parameters to the confirmation of molecular structure

Y Liu, A Navarro-Vázquez, RR Gil, C Griesinger… - Nature protocols, 2019 - nature.com
The use of anisotropic NMR data, such as residual dipolar couplings (RDCs) and residual
chemical shift anisotropies (RCSAs), has emerged as a powerful technique for structural …