Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
Y Wu, D Battalapalli, MJ Hakeem, V Selamneni… - Journal of …, 2021 - Springer
Antibiotic resistance is spreading rapidly around the world and seriously impeding efforts to
control microbial infections. Although nucleic acid testing is widely deployed for the …
control microbial infections. Although nucleic acid testing is widely deployed for the …
DNA in nanochannels: theory and applications
Nanofluidic structures have over the last two decades emerged as a powerful platform for
detailed analysis of DNA on the kilobase pair length scale. When DNA is confined to a …
detailed analysis of DNA on the kilobase pair length scale. When DNA is confined to a …
Optical DNA mapping in nanofluidic devices: principles and applications
V Müller, F Westerlund - Lab on a Chip, 2017 - pubs.rsc.org
Optical DNA mapping has over the last decade emerged as a very powerful tool for
obtaining long range sequence information from single DNA molecules. In optical DNA …
obtaining long range sequence information from single DNA molecules. In optical DNA …
Single-molecule optical genome mapping in nanochannels: multidisciplinarity at the nanoscale
J Jeffet, S Margalit, Y Michaeli… - Essays in …, 2021 - portlandpress.com
The human genome contains multiple layers of information that extend beyond the genetic
sequence. In fact, identical genetics do not necessarily yield identical phenotypes as evident …
sequence. In fact, identical genetics do not necessarily yield identical phenotypes as evident …
Molecular Epidemiology of OXA-48 and NDM-1 Producing Enterobacterales Species at a University Hospital in Tehran, Iran, Between 2015 and 2016
H Solgi, S Nematzadeh, CG Giske, F Badmasti… - Frontiers in …, 2020 - frontiersin.org
Carbapenem-resistant Enterobacterales (CRE) is an increasing problem worldwide. Here,
we examined the clonal relatedness of 71 non-repetitive CRE isolates collected in a …
we examined the clonal relatedness of 71 non-repetitive CRE isolates collected in a …
Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping
Bacterial plasmids are extensively involved in the rapid global spread of antibiotic
resistance. We here present an assay, based on optical DNA mapping of single plasmids in …
resistance. We here present an assay, based on optical DNA mapping of single plasmids in …
Bacteriophage strain typing by rapid single molecule analysis
A Grunwald, M Dahan, A Giesbertz… - Nucleic acids …, 2015 - academic.oup.com
Rapid characterization of unknown biological samples is under the focus of many current
studies. Here we report a method for screening of biological samples by optical mapping of …
studies. Here we report a method for screening of biological samples by optical mapping of …
Phosphorylated CtIP bridges DNA to promote annealing of broken ends
The early steps of DNA double-strand break (DSB) repair in human cells involve the MRE11-
RAD50-NBS1 (MRN) complex and its cofactor, phosphorylated CtIP. The roles of these …
RAD50-NBS1 (MRN) complex and its cofactor, phosphorylated CtIP. The roles of these …
Long-read single-molecule maps of the functional methylome
H Sharim, A Grunwald, T Gabrieli, Y Michaeli… - Genome …, 2019 - genome.cshlp.org
We report on the development of a methylation analysis workflow for optical detection of
fluorescent methylation profiles along chromosomal DNA molecules. In combination with …
fluorescent methylation profiles along chromosomal DNA molecules. In combination with …
Optofluidic systems enabling detection in real samples: A review
Optofluidics, understood as the synergistic combination between microfluidics and
photonics, has been at the forefront of the scientific research due to its outmatching …
photonics, has been at the forefront of the scientific research due to its outmatching …