Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most
general and adaptable single-molecule techniques. Despite the explosive growth in the …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …

Optical spectroscopy at the ultimate limit of a single molecule has grown over the past dozen
years into a powerful technique for exploring the individual nanoscale behavior of molecules …

Single-stranded DNA (ssDNA) is an essential intermediate in various DNA metabolic
processes and interacts with a large number of proteins. Due to its flexibility, the …

Fluorescent resonance energy transfer (FRET) is a powerful technique for studying
conformational distribution and dynamics of biological molecules. Some conformational …

In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which
can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence …

Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …

Many biologically important macromolecules undergo motions that are essential to their
function. Biophysical techniques can now resolve the motions of single molecules down to …

Dynamic structural changes of macromolecules undergoing biochemical reactions can be
studied using novel single molecule spectroscopy tools. Recent advances in applying such …