Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Intracellular aggregation of the human amyloid protein α-synuclein is causally linked to
Parkinson's disease. While the isolated protein is intrinsically disordered, its native structure …

The electron–electron double resonance (DEER) method, which provides distance
distributions between two spin labels, attached site specifically to biomolecules (proteins …

The understanding of biomolecular function is coupled to knowledge about the structure and
dynamics of these biomolecules, preferably acquired under native conditions. In this regard …

Distance measurement in the nanometer range by electron paramagnetic resonance
spectroscopy (EPR) in combination with site-directed spin labeling is a very powerful tool to …

Abstract Structure determination of biomacromolecules under in‐cell conditions is a relevant
yet challenging task. Electron paramagnetic resonance (EPR) distance measurements in …

Approaching protein structural dynamics and protein–protein interactions in the cellular
environment is a fundamental challenge. Owing to its absolute sensitivity and to its …