Single‐cell analysis using droplet microfluidics

K Matuła, F Rivello, WTS Huck - Advanced Biosystems, 2020 - Wiley Online Library
Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …

Scaling by shrinking: empowering single-cell'omics' with microfluidic devices

SM Prakadan, AK Shalek, DA Weitz - Nature Reviews Genetics, 2017 - nature.com
Recent advances in cellular profiling have demonstrated substantial heterogeneity in the
behaviour of cells once deemed'identical', challenging fundamental notions of …

Single-cell barcoding and sequencing using droplet microfluidics

R Zilionis, J Nainys, A Veres, V Savova, D Zemmour… - Nature protocols, 2017 - nature.com
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

M He, J Crow, M Roth, Y Zeng, AK Godwin - Lab on a Chip, 2014 - pubs.rsc.org
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially
when biopsy is difficult, costly, and sometimes not even an option. Tumor-derived exosomes …

Droplet microfluidics for high-throughput biological assays

MT Guo, A Rotem, JA Heyman, DA Weitz - Lab on a Chip, 2012 - pubs.rsc.org
Droplet microfluidics offers significant advantages for performing high-throughput screens
and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to …

Droplet based microfluidics

R Seemann, M Brinkmann, T Pfohl… - Reports on progress …, 2011 - iopscience.iop.org
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining
soft matter physics, biochemistry and microsystems engineering. Its applications range from …

Surfactants in droplet-based microfluidics

JC Baret - Lab on a Chip, 2012 - pubs.rsc.org
Surfactants are an essential part of the droplet-based microfluidic technology. They are
involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics

AM Foudeh, TF Didar, T Veres, M Tabrizian - Lab on a Chip, 2012 - pubs.rsc.org
Effective pathogen detection is an essential prerequisite for the prevention and treatment of
infectious diseases. Despite recent advances in biosensors, infectious diseases remain a …