Protein analysis by shotgun/bottom-up proteomics
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
MS1 ion current‐based quantitative proteomics: A promising solution for reliable analysis of large biological cohorts
The rapidly‐advancing field of pharmaceutical and clinical research calls for systematic,
molecular‐level characterization of complex biological systems. To this end, quantitative …
molecular‐level characterization of complex biological systems. To this end, quantitative …
Statistical protein quantification and significance analysis in label-free LC-MS experiments with complex designs
Background Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is
widely used for quantitative proteomic investigations. The typical output of such studies is a …
widely used for quantitative proteomic investigations. The typical output of such studies is a …
IonStar enables high-precision, low-missing-data proteomics quantification in large biological cohorts
Reproducible quantification of large biological cohorts is critical for clinical/pharmaceutical
proteomics yet remains challenging because most prevalent methods suffer from drastically …
proteomics yet remains challenging because most prevalent methods suffer from drastically …
Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry
Kinases play a prominent role in tumor development, pointing to the presence of specific
phosphorylation patterns in tumor tissues. Here, we investigate whether recently developed …
phosphorylation patterns in tumor tissues. Here, we investigate whether recently developed …
A straightforward and highly efficient precipitation/on-pellet digestion procedure coupled with a long gradient nano-LC separation and Orbitrap mass spectrometry for …
X Duan, R Young, RM Straubinger… - Journal of proteome …, 2009 - ACS Publications
For label-free expression profiling of tissue proteomes, efficient protein extraction, thorough
and quantitative sample cleanup and digestion procedures, as well as sufficient and …
and quantitative sample cleanup and digestion procedures, as well as sufficient and …
An IonStar experimental strategy for MS1 ion current-based quantification using ultrahigh-field orbitrap: reproducible, in-depth, and accurate protein measurement in …
In-depth and reproducible protein measurement in many biological samples is often critical
for pharmaceutical/biomedical proteomics but remains challenging. MS1-based …
for pharmaceutical/biomedical proteomics but remains challenging. MS1-based …
Protein quantification in label-free LC-MS experiments
T Clough, M Key, I Ott, S Ragg… - Journal of proteome …, 2009 - ACS Publications
The goal of many LC-MS proteomic investigations is to quantify and compare the
abundance of proteins in complex biological mixtures. However, the output of an LC-MS …
abundance of proteins in complex biological mixtures. However, the output of an LC-MS …
High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation
Robust, reliable quantification of large sample cohorts is often essential for meaningful
clinical or pharmaceutical proteomics investigations, but it is technically challenging. When …
clinical or pharmaceutical proteomics investigations, but it is technically challenging. When …
[HTML][HTML] The human leukocyte antigen class II immunopeptidome of the SARS-CoV-2 spike glycoprotein
MD Knierman, MB Lannan, LJ Spindler, CL McMillian… - Cell Reports, 2020 - cell.com
Precise elucidation of the antigen sequences for T cell immunosurveillance greatly
enhances our ability to understand and modulate humoral responses to viral infection or …
enhances our ability to understand and modulate humoral responses to viral infection or …