The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …

Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …

Chromosome structure in mammals is thought to regulate transcription by modulating three-
dimensional interactions between enhancers and promoters, notably through CTCF …

The piggyBac DNA transposon is used widely in genome engineering applications. Unlike
other transposons, its excision site can be precisely repaired without leaving footprints and it …

Many genes are regulated by multiple enhancers that often simultaneously activate their
target gene. However, how individual enhancers collaborate to activate transcription is not …

DNA transposons offer an efficient nonviral method of permanently modifying the genomes
of mammalian cells. The piggyBac transposon system has proven effective in genomic …

Genetic perturbation screens have the potential to dissect a wide range of cellular
phenotypes. Such screens have historically been difficult in diploid mammalian cells. The …

Gene therapy protocols require robust and long-term gene expression. For two decades,
retrovirus family vectors have offered several attractive properties as stable gene-delivery …

The use of T cells from healthy donors for allogeneic chimeric antigen receptor T (CAR-T)
cell cancer therapy is attractive because healthy donor T cells can produce versatile off-the …

While multiple technologies for small allele genome editing exist, robust technologies for
targeted integration of large DNA fragments in mammalian genomes are still missing. Here …