Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Analytical characterization of liposomes and other lipid nanoparticles for drug delivery

Y Fan, M Marioli, K Zhang - Journal of pharmaceutical and biomedical …, 2021 - Elsevier
Lipid nanoparticles, especially liposomes and lipid/nucleic acid complexed nanoparticles
have shown great success in the pharmaceutical industry. Their success is attributed to …

FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls

WR Algar, N Hildebrandt, SS Vogel, IL Medintz - Nature methods, 2019 - nature.com
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …

Native mass spectrometry of membrane proteins

JE Keener, G Zhang, MT Marty - Analytical chemistry, 2020 - ACS Publications
Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …

[HTML][HTML] Advanced fluorescence microscopy techniques—Frap, Flip, Flap, Fret and flim

HC Ishikawa-Ankerhold, R Ankerhold, GPC Drummen - Molecules, 2012 - mdpi.com
Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

DNA probes for monitoring dynamic and transient molecular encounters on live cell membranes

M You, Y Lyu, D Han, L Qiu, Q Liu, T Chen… - Nature …, 2017 - nature.com
Cells interact with the extracellular environment through molecules expressed on the
membrane. Disruption of these membrane-bound interactions (or encounters) can result in …

Lipid–protein interactions in plasma membrane organization and function

T Sych, KR Levental, E Sezgin - Annual review of biophysics, 2022 - annualreviews.org
Lipid–protein interactions in cells are involved in various biological processes, including
metabolism, trafficking, signaling, host–pathogen interactions, and transmembrane …

Investigating supramolecular systems using Förster resonance energy transfer

AJP Teunissen, C Pérez-Medina, A Meijerink… - Chemical Society …, 2018 - pubs.rsc.org
Supramolecular systems have applications in areas as diverse as materials science,
biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems …

[HTML][HTML] Liposomes characterization for market approval as pharmaceutical products: Analytical methods, guidelines and standardized protocols.

S Giordani, V Marassi, A Zattoni, B Roda… - … of Pharmaceutical and …, 2023 - Elsevier
Liposomes are nano-sized lipid-based vesicles widely studied for their drug delivery
capabilities. Compared to standard carries they exhibit better properties such as improved …

pH-responsive cationic liposome for endosomal escape mediated drug delivery

S Rayamajhi, J Marchitto, TDT Nguyen… - Colloids and Surfaces B …, 2020 - Elsevier
Endosomal degradation of the nanoparticle is one of the major biological barriers
associated with the drug delivery system. Nanoparticles are internalized in the cell via …