Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Analytical characterization of liposomes and other lipid nanoparticles for drug delivery
Y Fan, M Marioli, K Zhang - Journal of pharmaceutical and biomedical …, 2021 - Elsevier
Lipid nanoparticles, especially liposomes and lipid/nucleic acid complexed nanoparticles
have shown great success in the pharmaceutical industry. Their success is attributed to …
have shown great success in the pharmaceutical industry. Their success is attributed to …
FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …
However, different FRET techniques are not applied consistently, nor are results uniformly …
Native mass spectrometry of membrane proteins
Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …
separate cells from their external environments and define subcellular compartments …
[HTML][HTML] Advanced fluorescence microscopy techniques—Frap, Flip, Flap, Fret and flim
HC Ishikawa-Ankerhold, R Ankerhold, GPC Drummen - Molecules, 2012 - mdpi.com
Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …
DNA probes for monitoring dynamic and transient molecular encounters on live cell membranes
Cells interact with the extracellular environment through molecules expressed on the
membrane. Disruption of these membrane-bound interactions (or encounters) can result in …
membrane. Disruption of these membrane-bound interactions (or encounters) can result in …
Lipid–protein interactions in plasma membrane organization and function
Lipid–protein interactions in cells are involved in various biological processes, including
metabolism, trafficking, signaling, host–pathogen interactions, and transmembrane …
metabolism, trafficking, signaling, host–pathogen interactions, and transmembrane …
Investigating supramolecular systems using Förster resonance energy transfer
Supramolecular systems have applications in areas as diverse as materials science,
biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems …
biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems …
[HTML][HTML] Liposomes characterization for market approval as pharmaceutical products: Analytical methods, guidelines and standardized protocols.
Liposomes are nano-sized lipid-based vesicles widely studied for their drug delivery
capabilities. Compared to standard carries they exhibit better properties such as improved …
capabilities. Compared to standard carries they exhibit better properties such as improved …
pH-responsive cationic liposome for endosomal escape mediated drug delivery
S Rayamajhi, J Marchitto, TDT Nguyen… - Colloids and Surfaces B …, 2020 - Elsevier
Endosomal degradation of the nanoparticle is one of the major biological barriers
associated with the drug delivery system. Nanoparticles are internalized in the cell via …
associated with the drug delivery system. Nanoparticles are internalized in the cell via …