Flow cytometry and cell sorting are well-established technologies in clinical diagnostics and
biomedical research. Heterogeneous mixtures of cells are placed in suspension and passed …

Due to its unique capability to analyze a large number of single cells for several parameters
simultaneously, flow cytometry has changed our understanding of the behavior of cells in …

Flow cytometry was partnered with a nonfluorescent reporter protein for rapid, early stage
identification of clones producing high levels of a therapeutic protein. A cell surface protein …

The success of engineered monoclonal antibodies as biopharmaceuticals has generated
considerable interest in strategies designed to accelerate development of antibody …

The plethora of genome sequences produced in the postgenomic age has not resolved
many of our most pressing biological questions. Correlating gene expression with an …

Background A majority of bacterial genes belong to tight clusters and operons, which
complicates gene functional studies using conventional knock-out methods. Antisense …

Cloning and expression plasmids are the workhorses of modern molecular biology. Despite
the pathway paved by synthetic biology, laboratories around the globe still relay on standard …

To unravel intricate mechanisms of gene regulation it is imperative to work in physiologically
relevant conditions and therefore preferentially in single copy constructs, which are not …

A red fluorescent protein, DsRed, which emits fluorescence in the red region of the spectrum
has become a popular alternative to green fluorescent protein as a label in biochemical and …

Fluorescence-based reporter systems are valuable tools for studying gene expression
dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the …