Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

P Patra, M Das, P Kundu, A Ghosh - Biotechnology Advances, 2021 - Elsevier
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories …

C Madzak - Journal of Fungi, 2021 - mdpi.com
Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast
Yarrowia lipolytica appears as one of the most attractive for a large range of white …

Comparison of yeasts as hosts for recombinant protein production

AM Vieira Gomes, T Souza Carmo, L Silva Carvalho… - Microorganisms, 2018 - mdpi.com
Recombinant protein production emerged in the early 1980s with the development of
genetic engineering tools, which represented a compelling alternative to protein extraction …

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

J Blazeck, A Hill, L Liu, R Knight, J Miller, A Pan… - Nature …, 2014 - nature.com
Economic feasibility of biosynthetic fuel and chemical production hinges upon harnessing
metabolism to achieve high titre and yield. Here we report a thorough genotypic and …

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

CM Schwartz, MS Hussain, M Blenner… - ACS synthetic …, 2016 - ACS Publications
The oleaginous yeast Yarrowia lipolytica is a valuable microbial host for chemical
production because it has a high capacity to synthesize, modify, and store intracellular lipids; …

Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica

Z Xue, PL Sharpe, SP Hong, NS Yadav, D Xie… - Nature …, 2013 - nature.com
The availability of the omega-3 fatty acids eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA) is currently limited because they are produced mainly by …

Metabolic engineering in the host Yarrowia lipolytica

AM Abdel-Mawgoud, KA Markham, CM Palmer… - Metabolic …, 2018 - Elsevier
The nonconventional, oleaginous yeast, Yarrowia lipolytica is rapidly emerging as a
valuable host for the production of a variety of both lipid and nonlipid chemical products …

Production of recombinant proteins by yeast cells

E Çelik, P Çalık - Biotechnology advances, 2012 - Elsevier
Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …

Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications

P Fickers, PH Benetti, Y Waché, A Marty… - FEMS yeast …, 2005 - academic.oup.com
The alkane-assimilating yeast Yarrowia lipolytica degrades very efficiently hydrophobic
substrates such as n-alkanes, fatty acids, fats and oils for which it has specific metabolic …

EasyCloneYALI: CRISPR/Cas9‐Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica

C Holkenbrink, MI Dam, KR Kildegaard… - Biotechnology …, 2018 - Wiley Online Library
The oleaginous yeast Yarrowia lipolytica is an emerging host for production of fatty acid‐
derived chemicals. To enable rapid iterative metabolic engineering of this yeast, there is a …