Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS)

MA Graewert, DI Svergun - Current opinion in structural biology, 2013 - Elsevier
Highlights•Popularity of SAXS/WAXS for structural analysis has gained substantially.•
Remarkable developments in hardware and software allow for high-throughput …

[HTML][HTML] Recent advances in X-ray compatible microfluidics for applications in soft materials and life sciences

A Ghazal, JP Lafleur, K Mortensen, JP Kutter, L Arleth… - Lab on a Chip, 2016 - pubs.rsc.org
The increasingly narrow and brilliant beams at X-ray facilities reduce the requirements for
both sample volume and data acquisition time. This creates new possibilities for the types …

[PDF][PDF] Improved radiation dose efficiency in solution SAXS using a sheath flow sample environment

N Kirby, N Cowieson, AM Hawley… - … Section D: Structural …, 2016 - journals.iucr.org
Radiation damage is a major limitation to synchrotron small-angle X-ray scattering analysis
of biomacromolecules. Flowing the sample during exposure helps to reduce the problem …

[HTML][HTML] Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses

M Reiser, A Girelli, A Ragulskaya, S Das… - Nature …, 2022 - nature.com
X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights
into structural dynamics of biological macromolecule solutions. However, very high dose …

Limiting radiation damage for high-brilliance biological solution scattering: practical experience at the EMBL P12 beamline PETRAIII

CM Jeffries, MA Graewert, DI Svergun… - Journal of synchrotron …, 2015 - journals.iucr.org
Radiation damage is the general curse of structural biologists who use synchrotron small-
angle X-ray scattering (SAXS) to investigate biological macromolecules in solution. The …

Imaging of biological materials and cells by X-ray scattering and diffraction

CYJ Hémonnot, S Köster - ACS nano, 2017 - ACS Publications
Cells and biological materials are large objects in comparison to the size of internal
components such as organelles and proteins. An understanding of the functions of these …

[HTML][HTML] Advances in integrative structural biology: Towards understanding protein complexes in their cellular context

SJ Ziegler, SJB Mallinson, PCS John… - Computational and …, 2021 - Elsevier
Microorganisms rely on protein interactions to transmit signals, react to stimuli, and grow.
One of the best ways to understand these protein interactions is through structural …

[HTML][HTML] Towards molecular movies with X-ray photon correlation spectroscopy

F Perakis, C Gutt - Physical Chemistry Chemical Physics, 2020 - pubs.rsc.org
In this perspective article we highlight research opportunities and challenges in probing
structural dynamics of molecular systems using X-ray Photon Correlation Spectroscopy …

[HTML][HTML] X-ray photon correlation spectroscopy of protein dynamics at nearly diffraction-limited storage rings

J Möller, M Sprung, A Madsen, C Gutt - IUCrJ, 2019 - scripts.iucr.org
This study explores the possibility of measuring the dynamics of proteins in solution using X-
ray photon correlation spectroscopy (XPCS) at nearly diffraction-limited storage rings …

High-pressure small-angle X-ray scattering cell for biological solutions and soft materials

DK Rai, RE Gillilan, Q Huang, R Miller… - Journal of Applied …, 2021 - journals.iucr.org
Pressure is a fundamental thermodynamic parameter controlling the behavior of biological
macromolecules. Pressure affects protein denaturation, kinetic parameters of enzymes …