Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

A true circular carbon economy must upgrade waste greenhouse gases. C1-based
biomanufacturing is an attractive solution, in which one carbon (C1) molecules (eg CO2 …

The past decade has seen growing interest in bacterial engineering for therapeutically
relevant applications. While early efforts focused on repurposing genetically tractable model …

Biocatalysis has emerged as a promising alternative to conventional chemical processes for
the production of a wide range of chemicals, providing a sustainable solution to the problem …

Eubacterium limosum is a Clostridial acetogen that efficiently utilizes a wide range of single-
carbon substrates and contributes to metabolism of health-associated compounds in the …

Abstract Background The genus Eubacterium is quite diverse and includes several
acetogenic strains capable of fermenting C1-substrates into valuable products. Especially …

Bacteriophages constitute one of the largest sources of unknown gene content in the
biosphere. Even for well-studied model phages, robust experimental approaches to identify …

In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …

The strain KIST612, initially identified as E. limosum, was a suspected member of E.
callanderi due to differences in phenotype, genotype, and average nucleotide identity (ANI) …

Clustered regularly interspaced short palindromic repeats (CRISPR) has revolutionized the
field of genome editing. To circumvent the permanent modifications made by traditional …