Differential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical
technique that has seen many applications over the years, ranging from protein folding state …

Phosphorylation is an essential process in biological events and is considered critical for
biological functions. In tissues, protein phosphorylation mainly occurs on tyrosine (Tyr) …

Thermal shift assays are used to study thermal stabilization of proteins upon ligand binding.
Such assays have been used extensively on purified proteins in the drug discovery industry …

Purification of recombinant proteins for biochemical assays and structural studies is time‐
consuming and presents inherent difficulties that depend on the optimization of protein …

The efficacy of therapeutics is dependent on a drug binding to its cognate target.
Optimization of target engagement by drugs in cells is often challenging, because drug …

Abstract Targeting the PD-1/PD-L1 immunologic checkpoint with monoclonal antibodies has
provided unprecedented results in cancer treatment in the recent years. Development of …

Stereochemically and structurally complex cyclic dinucleotide-based stimulator of interferon
genes (STING) agonists were designed and synthesized to access a previously unexplored …

Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to
identify low-molecular-weight ligands that bind and stabilize purified proteins. The …

Inorganic anions inhibit the metalloenzyme carbonic anhydrase (CA, EC 4.2. 1.1) generally
by coordinating to the active site metal ion. Cyanate was reported as a non-coordinating CA …

A drug must engage its intended target to achieve its therapeutic effect. However,
conclusively measuring target engagement (TE) in situ is challenging. This complicates …