A major goal of evolutionary genetics is to understand the genetic processes that give rise to
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …

Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …

Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

Molecular biology has been led by various measurement technologies, and increased
throughput has developed omics analysis. The development of massively parallel …

Sequencing the RNA in a biological sample can unlock a wealth of information, including
the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional …

Splicing varies across brain regions, but the single-cell resolution of regional variation is
unclear. We present a single-cell investigation of differential isoform expression (DIE) …

While splicing changes caused by somatic mutations in SF3B1 are known, identifying full-
length isoform changes may better elucidate the functional consequences of these …

A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …

Background: Given the demonstrated utility of Third Generation Sequencing [Pacific
Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many …