Covering: mid 1990s to 2018 Over the last two decades, diverse approaches have been
explored to generate new polyketides by engineering polyketide synthases (PKSs) …

Metabolic engineering efforts toward rewiring metabolism of cells to produce new
compounds often require the utilization of non-native enzymatic machinery that is capable of …

Type I modular polyketide synthases (PKSs) are polymerases that utilize acyl-CoAs as
substrates. Each polyketide elongation reaction is catalyzed by a set of protein domains …

In nature, various enzymes govern diverse biochemical reactions through their specific three-
dimensional structures, which have been harnessed to produce many useful bioactive …

ClusterCAD is a web-based toolkit designed to leverage the collinear structure and
deterministic logic of type I modular polyketide synthases (PKSs) for synthetic biology …

Highlights•Microbial polyketide (PK) pathway provides diverse biofuels and industrial
chemicals.•Fine-tuned production of valuable chemicals requires rational engineering of …

The biosynthesis of reduced polyketides in bacteria by modular polyketide synthases (PKSs)
proceeds with exquisite stereocontrol. As the stereochemistry is intimately linked to the …

Engineering of assembly line polyketide synthases (PKSs) to produce novel bioactive
compounds has been a goal for over 20 years. The apparent modularity of PKSs has …

Natural products are promising chemicals due to their structural diversity and bioactivities.
Over the decades, a vast variety of gene clusters encoding natural products have been …

14-16 元环的大环内酯类抗生素(Macrolide antibiotics, MA) 是临床上重要的抗感染药物.
随着细菌耐药性的不断增加, 迫切需要研发出新型MA 来应对耐药菌. 通过MA …