A review on recent advances for nucleants and nucleation in protein crystallization

RB Zhou, HL Cao, CY Zhang, DC Yin - CrystEngComm, 2017 - pubs.rsc.org
The elucidation of protein structures by X-ray crystallography remains the most effectual
method to provide accurate structural details at atomic resolution for rational drug design …

Protein crystallisation with air bubble templates: case of gas–liquid–solid interfaces

W Tian, C Rielly, H Yang - CrystEngComm, 2021 - pubs.rsc.org
Heterogeneous surfaces, such as solid particles, are known to efficiently increase nucleation
rates during crystallisations from solution. In this work, air bubbles have been used to act as …

A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity

E Pechkova, NL Bragazzi… - Critical Reviews™ in …, 2014 - dl.begellhouse.com
Crystallization is a highly demanding and time-consuming task that causes a real bottle-
neck in basic research. Great effort has been made to understand the factors and …

Langmuir–Blodgett nanotemplates for protein crystallography

E Pechkova, C Nicolini - Nature Protocols, 2017 - nature.com
The new generation of synchrotrons and microfocused beamlines has enabled great
progress in X-ray protein crystallography, resulting in new 3D atomic structures for proteins …

Advances in nanocrystallography as a proteomic tool

E Pechkova, NL Bragazzi, C Nicolini - Advances in Protein Chemistry and …, 2014 - Elsevier
In order to overcome the difficulties and hurdles too much often encountered in crystallizing
a protein with the conventional techniques, our group has introduced the innovative …

Ab Initio Semi-Quantitative Analysis of Micro-Beam Grazing-Incidence Small-Angle X-Ray Scattering (Μ-GISAXS) during Protein Crystal Nucleation and Growth

C Nicolini, NL Bragazzi, E Pechkova… - Journal of Proteomics …, 2014 - hal.science
Micro-beam Grazing-Incidence Small-Angle X-ray scattering (μ-GISAXS), exploiting both the
advantages of elastic X-ray scattering and the highly focused third-generation synchrotron …

[PDF][PDF] In situ monitoring by raman spectroscopy of lysozyme conformation during “nanotemplate” induced crystallization

C Nicolini, L Belmonte, G Maksimov… - J Microb Biochem …, 2013 - academia.edu
Using Raman spectroscopy and lysozyme, this latter as model protein, we investigate the
differences in protein conformation before and after LB nanotemplate-induced crystal …

[HTML][HTML] Protein crystallization by anodic porous alumina (apa) template: The example of hen egg white lysozyme (hewl)

E Pechkova, NL Bragazzi, C Nicolini - NanoWorld Journal, 2015 - jnanoworld.com
In this communication, we report anodic porous alumina (APA) template induced
crystallization. The APA nanotemplate was prepared on the glass substrate for the hen egg …

Langmuir-Blodgett (LB)-based Nanobiocrystallography at the Frontiers of Cancer Proteomics

E Pechkova, NL Bragazzi, S Fiordoro… - Anticancer …, 2015 - ar.iiarjournals.org
Background/Aim: Langmuir–Blodgett (LB) films used as templates for crystallization lead to
marked changes in protein stability and water dehydration, despite slight changes in protein …

[引用][C] Raman spectroscopy of protein crystal nucleation and growth

P Eugenia, S Fiordoro, N Claudio, P Evgenia… - American Journal of …, 2014 - elibrary.ru