Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications

K Kachhawaha, S Singh, K Joshi, P Nain… - Preparative …, 2023 - Taylor & Francis
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

[HTML][HTML] Current strategies to improve yield of recombinant protein production in rice suspension cells

YH Chi, LF Huang - Processes, 2022 - mdpi.com
A plant cell-based recombinant glucocerebrosidase was approved by the FDA in 2012 for
the treatment of human inherited Gaucher disease, indicating that plant suspension cells …

Protein Extraction and Purification by Differential Solubilization

BJ Ryan, GK Kinsella, GT Henehan - Protein Chromatography: Methods …, 2023 - Springer
The preparation of purified soluble proteins for biochemical studies is essential and the
solubility of a protein of interest in various media is central to this process. Selectively …

Utilization of freeze-thawing method for high-level expression of functional human epidermal growth factor (hEGF)

M Mohammadi, S Ghanbari, A Emamgholi… - International Journal of …, 2023 - Springer
The wide applications of human epidermal growth factor (hEGF) as an efficient mitogen
agent in the clinical, health, and cosmetic industries has made it an interesting research …

Rational design of soluble expressed human aldehyde dehydrogenase 2 with high stability and activity in pepsin and trypsin

M Hu, JX Song, ST Miao, CK Wu, XW Gong… - International Journal of …, 2024 - Elsevier
Abstract Acetaldehyde dehydrogenase 2 (ALDH2) is a crucial enzyme in alcohol
metabolism, and oral administration of ALDH2 is a promising method for alcohol …

Heterologous expression of a deacetylase and its application in l-glufosinate preparation

YS Wang, MH Gong, JH Wang, JC Yu, MJ Li… - Bioprocess and …, 2023 - Springer
With potent herbicidal activity, biocatalysis synthesis of l-glufosinate has drawn attention. In
present research, NAP-Das2. 3, a deacetylase capable of stereoselectively resolving N …

[HTML][HTML] Heterologous Expression of Difficult to Produce Proteins in Bacterial Systems

N Ferrer-Miralles, E Garcia-Fruitós - International journal of molecular …, 2024 - mdpi.com
Proteins play a crucial role in maintaining homeostasis, providing structure, and enabling
various functions in biological systems. The study of proteins was a complex task before the …

Check Chapter 17 updates

BJ Ryan, GK Kinsella, GT Henehan - … : Methods and Protocols, 2023 - books.google.com
The preparation of purified soluble proteins for biochemical studies is essential and the
solubility of a protein of interest in various media is central to this process. Selectively …

Ultrastructural features of the body wall of the helminth Heterakis dispar (Schrank, 1790)(Nematoda, Heterakidae)

FH Rzayev - Parazitologiâ, 2023 - journals.rcsi.science
The structure of the body wall (cuticle, hypoderm, and muscle layer) of the nematode
Heterakis dispar (Schrank, 1790) from the family Heterakidae was studied using light and …

热纤梭菌耐热葡聚糖外切酶的克隆表达及酶学特性分析.

王珊珊, 刘阳, 任艳艳, 王令, 路宏朝… - Feed Research, 2024 - search.ebscohost.com
研究旨在开发耐热纤维素酶, 以提高畜禽对纤维素饲料的利用率和养殖经济效益.
从蛋白质信息数据库UniProt 中获取热纤梭菌(Clostridium thermocellum) 葡聚糖外切酶CelS …