Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

Inteins are auto-processing domains found in organisms from all domains of life. These
proteins carry out a process known as protein splicing, which is a multi-step biochemical …

To test whether distances derived from paramagnetic broadening of 15N heteronuclear
single quantum coherence (HSQC) resonances could be used to determine the global fold …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

Biomoleküle in ihrer natürlichen Umgebung zu erforschen, ist wegen der enormen
Komplexität zellulärer Systeme eine anspruchsvolle Aufgabe. In den letzten Jahren …

Nuclear-magnetic-resonance spectroscopy can determine the three-dimensional structure of
proteins in solution. However, its potential has been limited by the difficulty of interpreting …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme
(Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE …

We have studied the naturally split α subunit of the DNA polymerase III (DnaE) intein from
Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent …