Cryopreservation of gametes and embryos and their molecular changes

E Estudillo, A Jiménez, PE Bustamante-Nieves… - International Journal of …, 2021 - mdpi.com
The process of freezing cells or tissues and depositing them in liquid nitrogen at–196° C is
called cryopreservation. Sub-zero temperature is not a physiological condition for cells and …

Vitrification of human immature oocytes before and after in vitro maturation: a review

MA Khalili, A Shahedi, S Ashourzadeh… - Journal of assisted …, 2017 - Springer
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting
perspectives for fertility preservation where ovarian reserves are damaged by pathologies or …

Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

T García-Martínez, M Vendrell-Flotats… - International journal of …, 2020 - mdpi.com
This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the
in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to …

Cryopreservation of mammalian oocyte for conservation of animal genetics

JR Prentice, M Anzar - Veterinary medicine international, 2011 - Wiley Online Library
The preservation of the female portion of livestock genetics has become an international
priority; however, in situ conservation strategies are extremely expensive. Therefore, efforts …

A self-gelling hydrogel based on thiolated hyaluronic acid for three-dimensional culture of ovine preantral follicles

T Dahmardeh, MH Ghanian, B Ebrahimi - International Journal of Biological …, 2023 - Elsevier
Abstract Three-dimensional (3D) ovarian follicle culture offers a promising option for fertility
preservation in patients who cannot receive ovarian tissue transplantation. Our research …

Cryopreservation of human dental follicle tissue for use as a resource of autologous mesenchymal stem cells

BW Park, SJ Jang, JH Byun, YH Kang… - Journal of Tissue …, 2017 - Wiley Online Library
The main purpose of this study was to develop a cryopreservation method for human dental
follicle tissue to maintain autologous stem cells as a resource. A modified cryoprotectant …

IGF-1 treatment during in vitro maturation improves developmental potential of ovine oocytes through the regulation of PI3K/Akt and apoptosis signaling

PK Javvaji, A Dhali, JR Francis, AP Kolte… - Animal …, 2021 - Taylor & Francis
This study aimed to assess the effect of the insulin-like grow factor 1 (IGF-1) treatment during
in vitro maturation on the gene expression and developmental ability of ovine oocytes. Ovine …

Vitrification of bovine oocytes at different meiotic stages using the Cryotop method: assessment of morphological, molecular and functional patterns

JFW Sprícigo, K Morais, AR Ferreira, GM Machado… - Cryobiology, 2014 - Elsevier
This study aimed to investigate the functional, morphological and molecular patterns of
bovine oocytes vitrified at different times during in vitro maturation (IVM). Four groups of …

Effect of vitrification on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes

BS Rao, YU Mahesh, KV Charan, K Suman, N Sekhar… - Cryobiology, 2012 - Elsevier
The aim of the study was to evaluate meiotic maturation, and expression of genes coding for
oocyte secreted factors (GDF9, BMP15, TGFBR1, and BPR2) and apoptosis (BCL2, BAX …

[HTML][HTML] Human ovarian tissue vitrification/warming has minor effect on the expression of apoptosis-related genes

M Abdollahi, M Salehnia, S Salehpour… - Iranian Biomedical …, 2013 - ncbi.nlm.nih.gov
Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural
levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just …