Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …

Native mass spectrometry (nMS) is evolving into a workhorse for structural biology. The
plethora of online and offline preparation, separation, and purification methods as well as …

Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …

Mass spectrometry is well-established as one of the primary methods used to characterize
peptides and proteins. New advances in instrumentation and innovative analytical workflows …

Highlights•IM-MS provides valuable tools for structural characterization of protein
complexes.•The spread of conformational states adopted by a protein can be captured by IM …

With the recent success in determining membrane protein structures, further detailed
understanding of the identity and function of the bound lipidome is essential. Using an …

Progress in structural biology research has led to a high demand for powerful and yet
complementary analytical tools for structural characterization of proteins and protein …

METHODS Herein we describe alternative dissociation methods commonly employed within
mass spectrometry with an emphasis on the outcomes of probing native protein complexes …

Membrane proteins play critical biochemical roles but remain challenging to study. Recently,
native or nondenaturing mass spectrometry (MS) has made great strides in characterizing …

The last∼ 25 years has seen mass spectrometry (MS) emerge as an integral method in the
structural biology toolkit. In particular, MS has enabled the structural characterization of …