A practical guide to amplicon and metagenomic analysis of microbiome data
Advances in high-throughput sequencing (HTS) have fostered rapid developments in the
field of microbiome research, and massive microbiome datasets are now being generated …
field of microbiome research, and massive microbiome datasets are now being generated …
Mycobiome diversity: high-throughput sequencing and identification of fungi
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
Establishing microbial composition measurement standards with reference frames
Differential abundance analysis is controversial throughout microbiome research. Gold
standard approaches require laborious measurements of total microbial load, or absolute …
standard approaches require laborious measurements of total microbial load, or absolute …
Quantifying bias introduced by sample collection in relative and absolute microbiome measurements
DG Maghini, M Dvorak, A Dahlen, M Roos… - Nature …, 2024 - nature.com
To gain insight into the accuracy of microbial measurements, it is important to evaluate
sources of bias related to sample condition, preservative method and bioinformatic …
sources of bias related to sample condition, preservative method and bioinformatic …
Identifying biases and their potential solutions in human microbiome studies
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
Fungi in aquatic ecosystems
HP Grossart, S Van den Wyngaert, M Kagami… - Nature Reviews …, 2019 - nature.com
Fungi are phylogenetically and functionally diverse ubiquitous components of almost all
ecosystems on Earth, including aquatic environments stretching from high montane lakes …
ecosystems on Earth, including aquatic environments stretching from high montane lakes …
Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling
The use of relative abundance data from next generation sequencing (NGS) can lead to
misinterpretations of microbial community structures, as the increase of one taxon leads to …
misinterpretations of microbial community structures, as the increase of one taxon leads to …
Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap
T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …
Dynamic root microbiome sustains soybean productivity under unbalanced fertilization
M Wang, AH Ge, X Ma, X Wang, Q Xie, L Wang… - Nature …, 2024 - nature.com
Root-associated microbiomes contribute to plant growth and health, and are dynamically
affected by plant development and changes in the soil environment. However, how different …
affected by plant development and changes in the soil environment. However, how different …
A field guide for the compositional analysis of any-omics data
Background Next-generation sequencing (NGS) has made it possible to determine the
sequence and relative abundance of all nucleotides in a biological or environmental …
sequence and relative abundance of all nucleotides in a biological or environmental …