A practical guide to amplicon and metagenomic analysis of microbiome data

YX Liu, Y Qin, T Chen, M Lu, X Qian, X Guo… - Protein & cell, 2021 - academic.oup.com
Advances in high-throughput sequencing (HTS) have fostered rapid developments in the
field of microbiome research, and massive microbiome datasets are now being generated …

Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

Establishing microbial composition measurement standards with reference frames

JT Morton, C Marotz, A Washburne, J Silverman… - Nature …, 2019 - nature.com
Differential abundance analysis is controversial throughout microbiome research. Gold
standard approaches require laborious measurements of total microbial load, or absolute …

Quantifying bias introduced by sample collection in relative and absolute microbiome measurements

DG Maghini, M Dvorak, A Dahlen, M Roos… - Nature …, 2024 - nature.com
To gain insight into the accuracy of microbial measurements, it is important to evaluate
sources of bias related to sample condition, preservative method and bioinformatic …

Identifying biases and their potential solutions in human microbiome studies

JT Nearing, AM Comeau, MGI Langille - Microbiome, 2021 - Springer
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …

Fungi in aquatic ecosystems

HP Grossart, S Van den Wyngaert, M Kagami… - Nature Reviews …, 2019 - nature.com
Fungi are phylogenetically and functionally diverse ubiquitous components of almost all
ecosystems on Earth, including aquatic environments stretching from high montane lakes …

Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling

C Jian, P Luukkonen, H Yki-Järvinen, A Salonen… - PloS one, 2020 - journals.plos.org
The use of relative abundance data from next generation sequencing (NGS) can lead to
misinterpretations of microbial community structures, as the increase of one taxon leads to …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

Dynamic root microbiome sustains soybean productivity under unbalanced fertilization

M Wang, AH Ge, X Ma, X Wang, Q Xie, L Wang… - Nature …, 2024 - nature.com
Root-associated microbiomes contribute to plant growth and health, and are dynamically
affected by plant development and changes in the soil environment. However, how different …

A field guide for the compositional analysis of any-omics data

TP Quinn, I Erb, G Gloor, C Notredame… - …, 2019 - academic.oup.com
Background Next-generation sequencing (NGS) has made it possible to determine the
sequence and relative abundance of all nucleotides in a biological or environmental …