Pseudomonas aeruginosa is a Gram-negative bacterium belonging to the γ-proteobacteria.
Like other members of the Pseudomonas genus, it is known for its metabolic versatility and …

Membrane-spanning signaling pathways enable bacteria to alter gene expression in
response to extracytoplasmic stimuli. Many such pathways are cell-surface signaling (CSS) …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …

Flagellar synthesis is a highly regulated process in all motile bacteria. In Escherichia coli
and related species, the transcription factor FlhDC is the master regulator of a multi-tiered …

The opportunistic pathogen Pseudomonas aeruginosa is capable of establishing severe
and persistent infections in various eukaryotic hosts. It encodes a wide array of virulence …

Sigma factors are essential global regulators of transcription initiation in bacteria which
confer promoter recognition specificity to the RNA polymerase core enzyme. They provide …

The opportunistic pathogen Pseudomonas aeruginosa, like all members of the genus
Pseudomonas, has the capacity to thrive in very different environments, ranging from water …

Swarming surface motility is a complex adaptation leading to multidrug antibiotic resistance
and virulence factor production in Pseudomonas aeruginosa. Here, we expanded previous …

Studying parallel evolution of similar traits in independent within-species lineages provides
an opportunity to address evolutionary predictability of molecular changes underlying …

Despite intensive study for 50 years, the biochemical and genetic links between lysine
metabolism and central metabolism in Pseudomonas putida remain unresolved. To …