Protein lipoylation: an evolutionarily conserved metabolic regulator of health and disease
EA Rowland, CK Snowden, IM Cristea - Current opinion in chemical …, 2018 - Elsevier
Highlights•Lipoylation is a rare, but highly conserved lysine posttranslational modification.•
Lipoylated metabolic complexes are evolutionarily conserved from bacteria to humans.• …
Lipoylated metabolic complexes are evolutionarily conserved from bacteria to humans.• …
Mass spectrometry of protein complexes: from origins to applications
S Mehmood, TM Allison… - Annual Review of Physical …, 2015 - annualreviews.org
Now routine is the ability to investigate soluble and membrane protein complexes in the gas
phase of a mass spectrometer while preserving folded structure and ligand-binding …
phase of a mass spectrometer while preserving folded structure and ligand-binding …
[HTML][HTML] Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM
A Zhou, A Rohou, DG Schep, JV Bason… - elife, 2015 - elifesciences.org
Adenosine triphosphate (ATP), the chemical energy currency of biology, is synthesized in
eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a …
eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a …
[HTML][HTML] Collision cross sections for structural proteomics
Ion mobility mass spectrometry (IM-MS) allows the structural interrogation of biomolecules
by reporting their collision cross sections (CCSs). The major bottleneck for exploiting IM-MS …
by reporting their collision cross sections (CCSs). The major bottleneck for exploiting IM-MS …
Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase
J Zhao, S Benlekbir, JL Rubinstein - Nature, 2015 - nature.com
Abstract Eukaryotic vacuolar H+-ATPases (V-ATPases) are rotary enzymes that use energy
from hydrolysis of ATP to ADP to pump protons across membranes and control the pH of …
from hydrolysis of ATP to ADP to pump protons across membranes and control the pH of …
Approaches to heterogeneity in native mass spectrometry
AD Rolland, JS Prell - Chemical reviews, 2021 - ACS Publications
Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …
composition, and stoichiometry of biomolecules and their complexes from solution after they …
CIUSuite 2: next-generation software for the analysis of gas-phase protein unfolding data
Ion mobility–mass spectrometry (IM–MS) has become an important addition to the structural
biology toolbox, but separating closely related protein conformations remain challenging …
biology toolbox, but separating closely related protein conformations remain challenging …
The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes
E Boeri Erba, C Petosa - Protein Science, 2015 - Wiley Online Library
Mass spectrometry (MS) is a powerful tool for determining the mass of biomolecules with
high accuracy and sensitivity. MS performed under so‐called “native conditions”(native MS) …
high accuracy and sensitivity. MS performed under so‐called “native conditions”(native MS) …
[HTML][HTML] Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell
Ion mobility mass spectrometry experiments enable the characterization of mass, assembly,
and shape of biological molecules and assemblies. Here, a new radio-frequency confining …
and shape of biological molecules and assemblies. Here, a new radio-frequency confining …
[HTML][HTML] Mass spectrometry-enabled structural biology of membrane proteins
AN Calabrese, SE Radford - Methods, 2018 - Elsevier
The last∼ 25 years has seen mass spectrometry (MS) emerge as an integral method in the
structural biology toolkit. In particular, MS has enabled the structural characterization of …
structural biology toolkit. In particular, MS has enabled the structural characterization of …