Highlights•Lipoylation is a rare, but highly conserved lysine posttranslational modification.•
Lipoylated metabolic complexes are evolutionarily conserved from bacteria to humans.• …

Now routine is the ability to investigate soluble and membrane protein complexes in the gas
phase of a mass spectrometer while preserving folded structure and ligand-binding …

Adenosine triphosphate (ATP), the chemical energy currency of biology, is synthesized in
eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a …

Ion mobility mass spectrometry (IM-MS) allows the structural interrogation of biomolecules
by reporting their collision cross sections (CCSs). The major bottleneck for exploiting IM-MS …

Abstract Eukaryotic vacuolar H+-ATPases (V-ATPases) are rotary enzymes that use energy
from hydrolysis of ATP to ADP to pump protons across membranes and control the pH of …

Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …

Ion mobility–mass spectrometry (IM–MS) has become an important addition to the structural
biology toolbox, but separating closely related protein conformations remain challenging …

Mass spectrometry (MS) is a powerful tool for determining the mass of biomolecules with
high accuracy and sensitivity. MS performed under so‐called “native conditions”(native MS) …

Ion mobility mass spectrometry experiments enable the characterization of mass, assembly,
and shape of biological molecules and assemblies. Here, a new radio-frequency confining …

The last∼ 25 years has seen mass spectrometry (MS) emerge as an integral method in the
structural biology toolkit. In particular, MS has enabled the structural characterization of …