Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

Potatoes are developed vegetatively from tubers, and therefore potato virus transmission is
always a possibility. The potato leafroll virus (PLRV) is a highly devastating virus of the …

Plant diseases significantly impact the global economy, and plant pathogenic
microorganisms such as nematodes, viruses, bacteria, fungi, and viroids may be the etiology …

Potato virus S (PVS) is a noteworthy threat to the propagation of healthy seed potatoes.
Accurate and speedy detection is critical for effective PVS management. In the present study …

Thanks to its ease, speed, and sensitivity, CRISPR-based nucleic acid detection has been
increasingly explored for molecular diagnostics. However, one of its major limitations is lack …

Polymerase chain reaction is an approach to make numerous copies of specific DNA. PCR
has been applied for the investigation of infectious sicknesses caused by viral, protozoan …

Several isothermal techniques for the detection of plant pathogens have been developed
with the advent of molecular techniques. Among them, Recombinase Polymerase …

Abstract Extraintestinal pathogenic Escherichia coli (ExPEC) producing extended-spectrum
β-lactamases (ESBL) cause serious human infections due to their virulence and multidrug …