3D scaffolds to study basic cell biology

M Hippler, ED Lemma, S Bertels, E Blasco… - Advanced …, 2019 - Wiley Online Library
Mimicking the properties of the extracellular matrix is crucial for developing in vitro models of
the physiological microenvironment of living cells. Among other techniques, 3D direct laser …

Cell shape and forces in elastic and structured environments: from single cells to organoids

R Link, K Weißenbruch, M Tanaka… - Advanced Functional …, 2024 - Wiley Online Library
With the advent of mechanobiology, cell shape and forces have emerged as essential
elements of cell behavior and fate, in addition to biochemical factors such as growth factors …

[HTML][HTML] Multi-material multi-photon 3D laser micro-and nanoprinting

L Yang, F Mayer, UHF Bunz, E Blasco… - Light: Advanced …, 2021 - light-am.com
Abstract Three-dimensional (3D) laser micro-and nanoprinting based upon multi-photon
absorption has made its way from early scientific discovery to industrial manufacturing …

Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds

M Hippler, K Weißenbruch, K Richler, ED Lemma… - Science …, 2020 - science.org
Many essential cellular processes are regulated by mechanical properties of their
microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by …

Fiber Flexibility Reconciles Matrix Recruitment and the Fiber Modulus to Promote Cell Mechanosensing

X Dong, Q Sun, J Geng, X Liu, Q Wei - Nano Letters, 2024 - ACS Publications
The mechanical interaction between cells and the extracellular matrix is pervasive in
biological systems. On fibrous substrates, cells possess the ability to recruit neighboring …

Bimodal sensing of guidance cues in mechanically distinct microenvironments

ED Tabdanov, VV Puram, Z Win, A Alamgir… - Nature …, 2018 - nature.com
Contact guidance due to extracellular matrix architecture is a key regulator of carcinoma
invasion and metastasis, yet our understanding of how cells sense guidance cues is limited …

Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics

K Weißenbruch, J Grewe, M Hippler, M Fladung… - Elife, 2021 - elifesciences.org
Abstract Nonmuscle myosin II (NM II) is an integral part of essential cellular processes,
including adhesion and migration. Mammalian cells express up to three isoforms termed NM …

Extracellular matrix geometry and initial adhesive position determine stress fiber network organization during cell spreading

E Kassianidou, D Probst, J Jäger, S Lee, AL Roguet… - Cell reports, 2019 - cell.com
Three-dimensional matrices often contain highly structured adhesive tracks that require cells
to turn corners and bridge non-adhesive areas. Here, we investigate these complex …

Comparison of direct and inverse methods for 2.5 D traction force microscopy

JW Blumberg, US Schwarz - Plos one, 2022 - journals.plos.org
Essential cellular processes such as cell adhesion, migration and division strongly depend
on mechanical forces. The standard method to measure cell forces is traction force …

Geometrically defined environments direct cell division rate and subcellular YAP localization in single mouse embryonic stem cells

S Bertels, M Jaggy, B Richter, S Keppler, K Weber… - Scientific Reports, 2021 - nature.com
Mechanotransduction via yes-associated protein (YAP) is a central mechanism for decision-
making in mouse embryonic stem cells (mESCs). Nuclear localization of YAP is tightly …