[HTML][HTML] Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts

Y Lugani, R Rai, AA Prabhu, P Maan… - Biofuel Research …, 2020 - biofueljournal.com
Many countries have their biofuel policy programs in place as part of their overall strategy to
achieve sustainable development. Among biofuels, bioethanol as a promising alternative to …

Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context

V Liberato, C Benevenuti, F Coelho, A Botelho… - Catalysts, 2019 - mdpi.com
Clostridium sp. is a genus of anaerobic bacteria capable of metabolizing several substrates
(monoglycerides, diglycerides, glycerol, carbon monoxide, cellulose, and more), into …

Systematic design and in vitro validation of novel one-carbon assimilation pathways

X Yang, Q Yuan, H Luo, F Li, Y Mao, X Zhao, J Du… - Metabolic …, 2019 - Elsevier
The utilization of one-carbon (C 1) assimilation pathways to produce chemicals and fuels
from low-cost C 1 compounds could greatly reduce the substrate-related production costs …

Systems engineering of Escherichia coli for high-level glutarate production from glucose

Z Zhang, R Chu, W Wei, W Song, C Ye, X Chen… - Nature …, 2024 - nature.com
Glutarate is a key monomer in polyester and polyamide production. The low efficiency of the
current biosynthetic pathways hampers its production by microbial cell factories. Herein …

Using co-culture to functionalize Clostridium fermentation

Y Cui, KL Yang, K Zhou - Trends in Biotechnology, 2021 - cell.com
Clostridium fermentations have been developed for producing butanol and other value-
added chemicals, but their development is constrained by some limitations, such as …

Recent advances in constraint and machine learning-based metabolic modeling by leveraging stoichiometric balances, thermodynamic feasibility and kinetic law …

PF Suthers, CJ Foster, D Sarkar, L Wang… - Metabolic …, 2021 - Elsevier
Understanding the governing principles behind organisms' metabolism and growth
underpins their effective deployment as bioproduction chassis. A central objective of …

Integrating thermodynamic and enzymatic constraints into genome-scale metabolic models

X Yang, Z Mao, X Zhao, R Wang, P Zhang, J Cai… - Metabolic …, 2021 - Elsevier
Stoichiometric genome-scale metabolic network models (GEMs) have been widely used to
predict metabolic phenotypes. In addition to stoichiometric ratios, other constraints such as …

Ethanol tolerance in engineered strains of Clostridium thermocellum

DG Olson, MI Maloney, AA Lanahan… - … for Biofuels and …, 2023 - Springer
Clostridium thermocellum is a natively cellulolytic bacterium that is promising candidate for
cellulosic biofuel production, and can produce ethanol at high yields (75–80% of theoretical) …

In Vivo Thermodynamic Analysis of Glycolysis in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum Using 13C and 2H Tracers

TB Jacobson, TK Korosh, DM Stevenson, C Foster… - Msystems, 2020 - Am Soc Microbiol
Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic
anaerobic bacteria with complementary metabolic capabilities that utilize distinct glycolytic …

Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in Clostridium thermocellum

S Hon, T Jacobson, DM Stevenson… - Applied and …, 2022 - Am Soc Microbiol
Glycolysis is an ancient, widespread, and highly conserved metabolic pathway that converts
glucose into pyruvate. In the canonical pathway, the phosphofructokinase (PFK) reaction …