Automated pipelines for rapid evaluation during cryoEM data acquisition

JH Mendez, EYD Chua, M Paraan, CS Potter… - Current Opinion in …, 2023 - Elsevier
Cryo-electron microscopy (cryoEM) has become a popular method for determining high-
resolution structures of biomolecules. However, data processing can be time-consuming …

The complexities of ligand/receptor interactions: Exploring the role of molecular vibrations and quantum tunnelling

OR Pagán - BioEssays, 2024 - Wiley Online Library
Molecular vibrations and quantum tunneling may link ligand binding to the function of
pharmacological receptors. The well‐established lock‐and‐key model explains a ligand's …

Tomo Live: an on-the-fly reconstruction pipeline to judge data quality for cryo-electron tomography workflows

M Comet, PM Dijkman, R Boer Iwema… - Biological …, 2024 - journals.iucr.org
Data acquisition and processing for cryo-electron tomography can be a significant
bottleneck for users. To simplify and streamline the cryo-ET workflow, Tomo Live, an on-the …

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

A Sawh-Gopal, A Ishemgulova, EYD Chua… - JoVE (Journal of …, 2023 - jove.com
Advancements in cryo-electron microscopy (cryoEM) techniques over the past decade have
allowed structural biologists to routinely resolve macromolecular protein complexes to near …

Smart Leginon enables cryoEM screening automation across multiple grids

A Sawh-Gopal, A Ishemgulova, EYD Chua, MF Aragon… - bioRxiv, 2023 - biorxiv.org
CryoEM multi-grid screening is often a tedious process that demands hours of attention.
Here, this protocol shows how to set up standard Leginon collection and Smart Leginon …