ELISA in the multiplex era: potentials and pitfalls

PJ Tighe, RR Ryder, I Todd… - PROTEOMICS–Clinical …, 2015 - Wiley Online Library
Multiplex immunoassays confer several advantages over widely adopted singleplex
immunoassays including increased efficiency at a reduced expense, greater output per …

Highly multiparametric analysis by mass cytometry

O Ornatsky, D Bandura, V Baranov, M Nitz… - Journal of …, 2010 - Elsevier
This review paper describes a new technology, mass cytometry, that addresses applications
typically run by flow cytometer analyzers, but extends the capability to highly multiparametric …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

The evasive nature of drug efficacy: implications for drug discovery

S Galandrin, G Oligny-Longpré, M Bouvier - Trends in pharmacological …, 2007 - cell.com
The efficacy of a drug is generally determined by the drug's ability to promote a quantifiable
biological response. In the context of the classical receptor-occupancy theory, the efficacy is …

Fluorescence activated cell sorting via a focused traveling surface acoustic beam

Z Ma, Y Zhou, DJ Collins, Y Ai - Lab on a Chip, 2017 - pubs.rsc.org
Fluorescence activated cell sorting (FACS) has become an essential technique widely
exploited in biological studies and clinical applications. However, current FACS systems are …

Monoplex and multiplex immunoassays: approval, advancements, and alternatives

H Ahsan - Comparative clinical pathology, 2022 - Springer
Immunoassays are a powerful diagnostic tool and are widely used for the quantification of
proteins and biomolecules in medical diagnosis and research. Enzyme-linked …

Spectral flow cytometry

JP Nolan, D Condello - Current protocols in cytometry, 2013 - Wiley Online Library
Interest in measuring the complete fluorescence spectra of individual cells in flow can be
traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and …

Multiplexed immunobead‐based assay for detection of oral cancer protein biomarkers in saliva

ME Arellano‐Garcia, S Hu, J Wang, B Henson… - Oral …, 2008 - Wiley Online Library
Objective: For clinical applications of biomarkers, there is a need for multiplex assays using
high throughput platforms. The objective of this study was to determine the efficacy of …

High-throughput, sensitive, and accurate multiplex PCR-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses

WM Lee, K Grindle, T Pappas, DJ Marshall… - Journal of clinical …, 2007 - Am Soc Microbiol
Human respiratory viruses are a diverse group of pathogens composed of hundreds of virus
strains, and this presents a major challenge for diagnostic laboratories. To efficiently detect …

[PDF][PDF] Can LC and LC-MS ever replace immunoassays?

TG Cross, MP Hornshaw - Journal of Applied Bioanalysis, 2016 - jab.scholasticahq.com
This review is not intended as a summary of research and development of immunoassays
and liquid chromatography-mass spectrometry but rather as a brief summary of the pros and …