Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Commonly used monomeric blue fluorescent proteins suffer from moderate brightness. The
brightest of them, mTagBFP, has a notably low chemical stability over time. Prolonged …

Fluorescence has long been recognized as a powerful tool for probing biological structure
and function. Nearly sixty-five years ago, Coons and colleagues introduced the first …

Recent progress of bioanalytical techniques has accelerated the deep understanding of
cellular states and development of novel drugs as well as medical diagnosis. Particularly …

1. INTRODUCTION The discovery of Anthozoa homologues of the green fluorescent protein
(GFP) from jellyfish Aequorea victoria, which emit not only green but also yellow, orange …

In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

The site-specific incorporation of three new coumarin lysine analogues into proteins was
achieved in bacterial and mammalian cells using an engineered pyrrolysyl-tRNA synthetase …

In the past few years a large series of the advanced red‐shifted fluorescent proteins (RFPs)
has been developed. These enhanced RFPs provide new possibilities to study biological …

The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have
enabled the generation of a growing palette of colors, providing unique opportunities for …