The phosphorus NMR spectra of intracellular ATP in glycolyzing human red blood cells
maintained at 37 degrees C in an atmosphere containing 5% CO2 have been obtained and …

Publisher Summary This chapter discusses the multiple functions and the metabolism of 2, 3-
bisphosphoglycerate (2, 3-P 2-glycerate). 2, 3-P 2-glycerate is widely present in living cells …

The effects of varying pH and of increasing intracellular hemoglobin (Hb) concentration on
red cell sickling and oxygen affinity were studied in whole blood from persons with sickle cell …

Hardly any molecule has attracted the interest of as many scientific disciplines as has
haemoglobin. Contributions from physicists, chemists and biologists have combined to bring …

A general model of co-operativity is developed and solved explicitly; it combines the
concepts of preferential binding and quaternary constraints (due to Monod, Wyman & …

Evidence is presented that a generalized concerted transition model provides a quantitative
understanding of (a) the molecular species that are present in solutions of partially liganded …

The intracellular distribution of ATP, 2, 3‐bisphosphoglycerate (P2‐glycerate) and Mg2+
was calculated for the oxygenated and deoxygenated human erythrocyte for the normal …

MATERIALS AND METHODS Oxyhemoglobin was prepared from blood freshly drawn and
purified by standard procedures(7). The protein was concentrated in collodion bags by …

Under simulated intracellular conditions (pH 7.2, 37° C, 130 mM KCl, 20 mM NaCl and
haemoglobin concentrations> 1.5 mM) the conditional association constants for the binding …

The influence of the red cell concentration of 2, 3-diphosphoglycerate (2, 3-DPG, 0.5–26
μmoles/g erythrocytes) on the “CO 2-Bohr effect”(pH varied by CO 2 at constant base …