FtsZ, a bacterial homolog of tubulin, is well established as forming the cytoskeletal
framework for the cytokinetic ring. Recent work has shown that purified FtsZ, in the absence …

Macromolecular crowding affects the activity of proteins and functional macromolecular
complexes in all cells, including bacteria. Crowding, together with physicochemical …

Constructing a minimal machinery for autonomous self-division of synthetic cells is a major
goal of bottom-up synthetic biology. One paradigm has been the E. coli divisome, with the …

Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins.
Monitoring the sedimentation of macromolecules in the centrifugal field allows their …

Binary fission of many prokaryotes as well as some eukaryotic organelles depends on the
FtsZ protein, which self-assembles into a membrane-associated ring structure early in the …

Membrane‐less organelles in cells are large, dynamic protein/protein or protein/RNA
assemblies that have been reported in some cases to have liquid droplet properties …

This paper overviews solution additives that affect protein stability and aggregation during
refolding, heating, and freezing processes. Solution additives are mainly grouped into two …

The Min biochemical network regulates bacterial cell division and is a prototypical example
of self-organizing molecular systems. Cell-free assays relying on purified proteins have …

The ever increasing problem of antibiotic resistance necessitates a search for new drug
molecules that would target novel proteins in the prokaryotic system. FtsZ is one such target …

▪ Abstract FtsZ is a prokaryotic tubulin homolog that assembles into a ring at the future site of
cell division. The resulting “Z ring” forms the framework for the division apparatus, and its …