The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

Homing endonucleases are encoded by open reading frames that are embedded within
group I, group II and archael introns, as well as inteins (intervening sequences that are …

Genetic methods of manipulating or eradicating disease vector populations have long been
discussed as an attractive alternative to existing control measures because of their potential …

Homing endonucleases are microbial DNA-cleaving enzymes that mobilize their own
reading frames by generating double strand breaks at specific genomic invasion sites …

Chromosomal rearrangements, including translocations, require formation and joining of
DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are …

Homing endonucleases confer mobility to their host intervening sequence, either an intron
or intein, by catalyzing a highly specific double-strand break in a cognate allele lacking the …

Self-renewal is the hallmark feature both of normal stem cells and cancer stem cells. Since
the regenerative capacity of normal haematopoietic stem cells is limited by the accumulation …

Meganucleases are sequence-specific endonucleases recognizing large (> 12 bp)
sequence sites and several laboratories have used these proteins to induce highly efficient …

The crystal structure of human cystatin C, a protein with amyloidogenic properties and a
potent inhibitor of cysteine proteases, reveals how the protein refolds to produce very tight …

Recognition and biochemical processing of DNA requires that proteins and other ligands
are able to distinguish their DNA binding sites from other parts of the molecule. In addition to …