Single-cell genomics has now made it possible to create a comprehensive atlas of human
cells. At the same time, it has reopened definitions of a cell's identity and of the ways in …

Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods
to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing …

Advances in the depth and quality of transcriptome sequencing have revealed many new
classes of long noncoding RNAs (lncRNAs). lncRNA classification has mushroomed to …

A revolution in cellular measurement technology is under way: For the first time, we have the
ability to monitor global gene regulation in thousands of individual cells in a single …

We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant,
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription …

The genetic, functional or compositional heterogeneity of healthy and diseased tissues
presents major challenges in drug discovery and development. Such heterogeneity hinders …

The development of new drugs is multidisciplinary and systematic work. High-throughput
techniques based on “-omics” have driven the discovery of biomarkers in diseases and …

INTRODUCTION The measurement of specific proteins and transcripts in individual cells is
critical for understanding the role of cellular diversity in development, health, and disease …

The discovery of various noncoding RNAs (ncRNAs) and their biological implications is a
growing area in cell biology. Increasing evidence has revealed canonical and noncanonical …