Monomer‐scale design of functional protein polymers using consensus repeat sequences

MP Chang, W Huang, DJ Mai - Journal of Polymer Science, 2021 - Wiley Online Library
Protein‐based polymers possess chemically defined sequences that can encode diverse
properties and functions into a new class of biopolymeric materials. However, sequence …

Bioorthogonal protein-DNA conjugation methods for force spectroscopy

M Synakewicz, D Bauer, M Rief, LS Itzhaki - Scientific Reports, 2019 - nature.com
Accurate and stable site-specific attachment of DNA molecules to proteins is a requirement
for many single-molecule force spectroscopy techniques. The most commonly used method …

Unraveling the mechanics of a repeat-protein nanospring: From folding of individual repeats to fluctuations of the superhelix

M Synakewicz, RS Eapen, A Perez-Riba… - ACS …, 2022 - ACS Publications
Tandem-repeat proteins comprise small secondary structure motifs that stack to form one-
dimensional arrays with distinctive mechanical properties that are proposed to direct their …

Engineering mono-and multi-valent inhibitors on a modular scaffold

A Diamante, PK Chaturbedy, PJE Rowling… - Chemical …, 2021 - pubs.rsc.org
Here we exploit the simple, ultra-stable, modular architecture of consensus-designed
tetratricopeptide repeat proteins (CTPRs) to create a platform capable of displaying both …

Exploring new strategies for grafting binding peptides onto protein loops using a consensus‐designed tetratricopeptide repeat scaffold

SK Madden, A Perez‐Riba, LS Itzhaki - Protein Science, 2019 - Wiley Online Library
Peptide display approaches, in which peptide epitopes of known binding activities are
grafted onto stable protein scaffolds, have been developed to constrain the peptide in its …

[HTML][HTML] Testing the length limit of loop grafting in a helical repeat protein

JF Ripka, A Perez-Riba, PK Chaturbedy… - Current Research in …, 2021 - Elsevier
Alpha-helical repeat proteins such as consensus-designed tetratricopeptide repeats
(CTPRs) are exceptionally stable molecules that are able to tolerate destabilizing sequence …

Decoupling a tandem-repeat protein: Impact of multiple loop insertions on a modular scaffold

A Perez-Riba, E Komives, ERG Main, LS Itzhaki - Scientific Reports, 2019 - nature.com
The simple topology and modular architecture of tandem-repeat proteins such as
tetratricopeptide repeats (TPRs) and ankyrin repeats makes them straightforward to dissect …

Exploring the binding of rationally engineered tandem-repeat proteins to E3 ubiquitin ligase Keap1

SK Madden, LS Itzhaki - Protein Engineering, Design and …, 2021 - academic.oup.com
The process of displaying functional peptides by 'grafting'them onto loops of a stable protein
scaffold can be used to impart binding affinity for a target, but it can be difficult to predict the …

Consensus tetratricopeptide repeat proteins are complex superhelical nanosprings

M Synakewicz, RS Eapen, A Perez-Riba, D Bauer… - bioRxiv, 2021 - biorxiv.org
Tandem-repeat proteins comprise small secondary structure motifs that stack to form one-
dimensional arrays with distinctive mechanical properties that are proposed to direct their …

Construction and Biological Characterisation of Custom Engineered Protein Degradation Tools.

S Stockton - 2022 - repository.cam.ac.uk
Event-driven pharmacology is a new paradigm in disease treatment for a wide range of
diseases, including cancer, that have failed by traditional occupancy-driven pharmacology …