The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation

H Brandhorst, A Friberg, HH Andersson, M Felldin… - …, 2009 - journals.lww.com
Background. The isolation of islets from the human pancreas critically depends on an
efficient enzyme blend. Previous studies have solely focused on the presence of …

Calcium: a crucial potentiator for efficient enzyme digestion of the human pancreas

T Eich, M Ståhle, B Gustafsson… - Cell …, 2018 - journals.sagepub.com
Background: Effective digestive enzymes are crucial for successful islet isolation.
Supplemental proteases are essential because they synergize with collagenase for effective …

Clostripain, the missing link in the enzyme blend for efficient human islet isolation

M Ståhle, A Foss, B Gustafsson, M Lempinen… - Transplantation …, 2015 - journals.lww.com
Background Effective digestive enzymes are crucial for successful islet isolation.
Supplemental proteases are essential as they synergize with collagenase for effective …

Pathogen inactivation of human serum facilitates its clinical use for islet cell culture and subsequent transplantation

MU Ståhle, D Brandhorst, O Korsgren… - Cell …, 2011 - journals.sagepub.com
Serum is regarded as an essential supplement to promote survival and growth of cells
during culture. However, the potential risk of transmitting diseases disqualifies the use of …

[HTML][HTML] How to use image analysis for islet counting

P Girman, Z Berkova, E Dobolilova… - The Review of Diabetic …, 2008 - ncbi.nlm.nih.gov
AIM: Assessment of islet mass before islet transplantation requires a reliable technique to
enable exact analysis of islet volume. This study aimed to test the applicability of digital …

Standardization of Islet Isolation and Transplantation Variables

AS Friberg - 2011 - diva-portal.org
Diabetes is a widespread and increasingly prevalent disease. Currently,-cell replacement
therapy via transplantation of islets of Langerhans is a viable means to maintain control of …

Technical challenges in human islet isolation

M Ståhle - 2015 - diva-portal.org
Background: Effective digestive enzymes are crucial for successful islet isolation.
Supplemental proteases are essential as they synergize with collagenase for effective …

[HTML][HTML] Original Data

P Girman, Z Berkova, E Dobolilova, F Saudek - Rev Diabet Stud, 2008 - soc-bdr.org
AIM: Assessment of islet mass before islet transplantation requires a reliable technique to
enable exact analysis of islet volume. This study aimed to test the applicability of digital …